in situ pcr meaning in Chinese
原位聚合酶链式反应
Examples
- It still remains a question whether the rearrangements of igh come from h / rs cell or the background lymphocytes . in this study , we have detected the igh clonal correlation between the h / rs cells and the background cells , from a new aspect to study the clonality of h / rs cell and its relation with the background cells . the expression of b - cell - specific activator protein ( bsap ) was detected in hl . igh gene rearrangements were analysed by the methods including gene analysis in neoplasms tissue and micropicked cells from paraffin - embedded sections , sequencing to test the pcr product , and in situ pcr
本研究将在以往研究的基础上，在国内率先把b细胞核反式作用因子? b细胞特异性激活蛋白( b - cell - specificactivatorprotein ， bsap )应用于hl的研究，检测hl的bsap表达，并采用石蜡刮片组织和微切割单细胞的基因分析、测序分析和间接原位pcr等方法，同步观察分析h rs和背景淋巴细胞的igh基因克隆相关性，从又一个新视角探究chl的肿瘤性h rs细胞克隆性及与背景淋巴细胞的关系。 - The organization cuts into slices and examines by the in situ pcr , drip protease k 20 ( xl with loomg / ml to digest respectively in pretreatment , increase with normal position positive cell account for total ratio of cell , according to the positive standard cells > 75 % , confirm the lightest digestion time , studying the influence and relationship of different fixation time with protease digesting each other , detecting the mn genotype of the organize slices at the same time
石蜡切片进行原位pcr检，预处理分别滴加loom岁血的蛋白酶k20闪消化，以原位扩增显色后阳性细胞占总细胞的比值> 75 %为标准，确定最适消化时间‘ ， ，研究不同固定时间与蛋白酶消化的相互影响和关系，同时检测石蜡切片的mn基因型。 - This research use high sensitive , special in situ pcr technology to determine the material which is treated by the paraffin wax and formalin fixation of different time , by detecting the genotype of mn blood group of the organizes slices . at the same time , we study the research of main influence factors , such as protease digestion , etc . we hope to set up a kind of steady , practical methods to detect the genotype of the material treated by paraffin wax and formlin , offer a kind of new detecting means for the forensic appraises and iditificition with individual material evidence
本研究应用灵敏度高、特异性好的原位pcr技术，测定福尔马林固定不同时间的石蜡切片组织mn血型的基因型，并对蛋白酶消化时间等主要影响因素进行了初步的研究，以建立一种稳定、实用的检测石蜡组织切片dna遗传标记的方法，为法医物证鉴定和个人识别提供一种新的检测手段。